MDL Form WGSR - Whole Genome Sequencing Test Request
Electronic Submittal Form Instructions
NOTE: We are unable to process samples with inadequate information.
1. On page one, under āSelect Test Requisitionā, use the pull-down menu to select Lab Form
āWhole Genome Sequencing Test Request-WGSRā. Provide all necessary information on page 1. For directions on how to fill out the fields on page 1, please refer to the ā
General Specimen Submission Page 1 Instructionsā document.
2. For WGSR ā Whole Genome Sequencing Test Request form, it is REQUIRED that the following fields be filled in:
2.1. Field 34 (Material Submitted).
2.2. Field 37 (Source).
2.3. Field 39 (Test(s) Requested).
2.4. Field 40 (Submitterās identification of Organisms).
3. Follow the instructions below to provide additional information on page 2 āWhole Genome Sequencing Test Request- WGSR - Additional Information":
3.1. In the box āTest requestedā, select the test requested (e.g. Primary analysis and Secondary analysis). If the test is requested by epidemiologists, please provide your name in the āRequested byā space and provide your e-mail address as well so that reports can be sent. Please consult with Core lab staff if you face difficulty in selecting the tests.
3.2. In the box āSample Typeā, please check the option āBacterial pure culture on solid mediaā. We only accept pure cultures or isolates at present. Please consult with Core lab staff if you wish to submit āBacterial DNAā.
3.3. If you are submitting multiple samples which belong to same species and are requesting for phylogenetic tree analysis, please select āBatch Analysisā.
4. Print the completed Lab Form WGSR front to back. The document is formatted for 2-sided printing.
5. Packaging and shipping: The submitter is responsible for making sure that all samples are packaged and shipped according to the current federal and state packaging and shipping regulations for āInfectious substance, Category Aā and/or āBiological substance, Category Bā.
6. Additional considerations when submitting samples:
6.1. Culture isolate should be pure.
6.2. Use a tubed solid agar medium that supports good growth of the organisms. Do not submit cultures on plated agar media.
6.3. Label sample tube with at least two identifiers (i.e. the patientās name, submitter lab number, date of birth) and the date inoculated.
6.4. Do not submit Enterobacteriaceae on TSI slants. The preferred medium is without carbohydrates.
6.5. For safety, all submitted culture tubes must have a tightened screw cap secured in place using tape.
6.6. Before shipment, insert the completed Lab Form WGSR and all attachments between the inner and outer shipping containers.
6.8. If you have any questions regarding sample submission, call the MDL for guidance ā 510-412-3700.