Test Order - Bacterial Bioterrorism Agent Identification or Confirmation
Pre-Approval Required
Yes
Supplemental Information or Form
1. Pure,
viable subculture
2. For
Brucella sp. confirmed to genus, a boiled undiluted cellular extract (see
description below) of a pure culture
3. Completed
submittal form with 2 patient identifiers (e.g. name and date of birth),
collection date and source, a brief but complete patient history, cultural
history and submitting laboratory’s findings
Performed on Specimens From
None
Acceptable Sample/Specimen Type for Testing
1. Bacterial
isolate
2. For
Brucella sp.confirmed to genus, a boiled undiluted cellular lysate of a
pure culture prepared using LRN extraction procedures with a final volume of 100
µl
is also acceptable
Minimum Volume Required
None
Storage & Preservation of Sample/Specimen Prior to Shipping
None
Transport
1. Bacterial
isolate: Tubed agar medium with a secure screw cap, labeled with patient’s
name
2. Boiled
undiluted cellular lysate: Minimum volume of 100
µl
in
a 1.5 to 2 ml taped screw cap conical centrifuge tube, labeled with the
patient’s name
Sample/Specimen Labeling
None
Shipping Instructions which Include Specimen Handling Requirements
For
Isolates:
1. Grow
isolate under appropriate conditions prior to shipping.
2. Ship
at ambient temperature using appropriate DOT/IATA approved shipping
procedures.
For
Cellular Extracts:
1. Ship
frozen or on a cold pack using appropriate DOT/IATA approved shipping
procedures.
Test Methodology
As
needed - Conventional Methods such as Biochemical testing, DFA, and phage lysis,
and PCR
Turnaround Time
None
Interferences & Limitations
Mixed
or nonviable culture
Additional Information
Please
contact the MDL High Risk Pathogens Section for special consideration if any of
the above criteria cannot be met.
MDL Points of Contact
High
Risk Pathogens Section (Select Agents) (510) 412-3903
fax number (510)
412-3706
Email: laura.smoot@cdph.ca.gov