Test Order - Carbapenemase Gene Detection
Pre-Approval Required
Isolates must be confirmed as Escherichia coli, Klebsiella oxytoca, Klebsiella pneumoniae, Klebsiella aerogenes, Enterobacter species or Pseudomonas aeruginosa.
At this time, acceptance of requests for testing of other Enterobacterales or Acinetobacter organisms is on a case by case basis after consultation with HAI program at HAIprogram@cdph.ca.gov or MDL points of contact listed below.
Isolates of Enterobacterales or Acinetobacter other than those listed above, that are submitted without prior consultation will not be tested.
Supplemental Information or Form
Form Lab AST
MDL Expanded Carbapenemase Testing Frequently Asked Questions (FAQ)
Performed on Specimens From
Human source
Acceptable Sample/Specimen Type for Testing
Pure culture isolate.
Isolates must be resistant to at least one carbapenem, i.e., imipenem, ertapenem, doripenem, or meropenem.
Isolates must be identified to at least the genus level by the submitter.
Mixed cultures received in MDL will not be tested.
Minimum Volume Required
None
Storage & Preservation of Sample/Specimen Prior to Shipping
Store
at ambient temperature
Transport
Ship
cultures on nonselective nutrient or similar agar in tubes with leak-proof screw
cap closures.
Sample/Specimen Labeling
Testing
subject to CLIA regulations require two patient identifiers on the specimen
container and the test requisition
Shipping Instructions which Include Specimen Handling Requirements
Ship
at ambient temperature.
Test Methodology
Carbapenemase Genotypic Detection: PCR based assay
Expanded AST (ExAST): Test provided through Antibiotic Resistance Laboratory Network (ARLN). Please see MDL Expanded Carbapenemase Testing Frequently Asked Questions (FAQ) (May 2021) for submission instruction.
Turnaround Time
Carbapenemase Genotypic Detection: 7 business days
Expanded AST (ExAST): Defined by ARLN testing laboratory
Interferences & Limitations
None
Additional Information
None
MDL Points of Contact
MDL ā Bacterial Diseases Section (510) 412-3903
Monday-Friday 8 AM to 5 PM PT
Email: MDL.Submissions@CDPH.ca.gov